The primary detector for the CGSC is a BioTek Synergy-2 microplate reader.
Our reader supports three major modes of readout,
- Flourescence
- Optical Absorbance
- Luminescence
Operating in Flourescence mode the reader can take four diffrent types of mesuerments,
- Intensity
- Polarisation
- FRET
- Time Resolved
A wide selection of filters and dichroics make most dyes and dye-pairs accessable to the reader.
Filters exist for: Tryptophan,DAPI,Fluorescein,GFP,EYFP,CY3,TRITC,Rhodamine,Texas Red,CY5.
At the moment Polarisation readings are limited to Fluorescein,GFP,CY3,Rhodamine,Texas Red,CY5.
If you would like to make use of another fluor please e-mail or call, we will likely be able to adjust our filteres, or get a new filter as part of your experiment.
Filters exist for: Tryptophan,DAPI,Fluorescein,GFP,EYFP,CY3,TRITC,Rhodamine,Texas Red,CY5.
At the moment Polarisation readings are limited to Fluorescein,GFP,CY3,Rhodamine,Texas Red,CY5.
If you would like to make use of another fluor please e-mail or call, we will likely be able to adjust our filteres, or get a new filter as part of your experiment.
Absorbance and Optical Density measuerments are made using a tuneable monochromator and a dedicated detector. Wavelengths from 280nm through 1000nm are accassable, Making pathlength corrections posible on a per-well bases. Optical densitys from 0.05 to 1.5 can be read with little or no distortion.
Highly sensitive Luminescence measuerments can be made using a speclized optical path. The reader is equiped with reagent injectors for flash luminescence readouts as well as glow based systems.