| epifluorescent | phase contrast |
|---|---|
| 4X PLAN APO 0.20 | 4X PLAN FLUOR DL 0.13 |
| 10X PLAN FLUOR 0.30 | 10X PH1 PLAN FLUOR DL 0.30 |
| 20X PLAN FLUOR ELWD 0.45 | 20X PH1 PLAN FLUOR ELWD DM 0.45 |
| 40X PLAN FLUOR ELWD 0.60 | 40X PH2 PLAN FLUOR ELWD DM 0.60 |
| 60X PLAN FLUOR ELWD 0.70 |   |
| Slot | Nominal Dye |
|---|---|
| 1 | DAPI/BFP |
| 2 | TRITC/Rhodamine |
| 3 | FITC/eGFP |
| 4 | Texas-Red/RFP/PI |
| 5 | Transmitted (none) |
In order to better support the use of high-content screening, the CGSC maintains the MetaExpress and AcuityExpress image anlysis and informatic packages.
Through the core computing infrastructure, users are able to access image processing applications for cell cycle, cell health, cell scoring, count nuclei, granularity, live/dead, mitoic index, neurite outgrowth, translocation, and angiogenesis tube formation.
Users may also request to have access to MetaExpress and AcuityExpress from there lab.
The core maintains a computing infrastructure to allow ease of processing data.
Central to this offline processing and data storage is a FiberChannel data storage system,
with over 4TB of space currently allocated to screening work.
Twin offline processing systems can automatically process the image data post-collection.
A central database server running Oracle-10g allows for informatics work using AcuityExpress.
Two user work stations in the CGSC allows users to begin data reduction while attending to ongoing data collections.