| epifluorescent | phase contrast |
|---|---|
| 4X PLAN APO 0.20 | 4X PLAN FLUOR DL 0.13 |
| 10X PLAN FLUOR 0.30 | 10X PH1 PLAN FLUOR DL 0.30 |
| 20X PLAN FLUOR ELWD 0.45 | 20X PH1 PLAN FLUOR ELWD DM 0.45 |
| 40X PLAN FLUOR ELWD 0.60 | 40X PH2 PLAN FLUOR ELWD DM 0.60 |
| 60X PLAN FLUOR ELWD 0.70 |   |
| Slot | Nominal Dye |
|---|---|
| 1 | DAPI/BFP |
| 2 | TRITC/Rhodamine |
| 3 | FITC/eGFP |
| 4 | Texas-Red/RFP/PI |
| 5 | Transmited(none) |
In oreder to better support the use of High-content screening the CGSC maintains the MetaExpress and AcuityExpress Image anlysis and informatic packages.
Through the core computing infrusctructer users are able to access image processing applications for, Cell Cycle, Cell Health, Cell Scoring, Count Nuclei, Granularity, Live/Dead, Mitoic Index, Neurite Outgrowth, Translocation, and Angiogenesis Tube Formation.
Users may also request to have access to MetaExpress and AcuityExpress from there lab.
The core maintains a computing infrustructer to allow ease of processing data,
Central to this offline processing and data storage is a FiberChannel data storage system,
with over 4TB of space currentaly allocated to screening work.
Twin offline processig systems that can automaticaly process the image data post collection.
A central Database server running Oracle-10g to allow for Informatics work using AcuityExpress.
As well as two user work stations in the Core so users can begin data reduction while attending to further data collections.