We provide plate-based assay services, screening resources, expertise, automation and libraries for both siRNA and small-molecule compound libraries suitable for cell-based and biochemical assays.
The High-Throughput Screening Core is the combination of two former screening cores: the CGSC and the HTC, and represents a collaboration between the Siteman Cancer Center and the Department of Biochemistry and Molecular Biophysics. This resource is available to the entire Washington University community.
- Experienced personnel work closely with researchers to develop and perform screens with siRNA or small molecules Support for any research that may benefit from automation.
- Access to instrumentation, including liquid-handling, plate readers, and high-content microscopy - individual instruments may be used offline for smaller studies
- Access to a compound collection of ~150,000 chemicals. A variety of sources and activities provides the capacity to screen relatively small bioactive libraries to develop assays or for proof-of-concept data, as well as for screening much larger libraries
- Access to siRNA collections, including Human Phosphatase, Kinase, Druggable Genome, Whole Genome, and Human and Mouse E3-ligase.
- Assay development (cell-based or biochemical)
- Provide access and training on instrumentation
- Library screening (internal library or external sources) and data analysis
- Provide access to the compound libraries: (ICCB known bioactives, NCI diversity set, Maybridge Hit finder, Microsource Spectrum Collection, Chembridge Microformats, Microsource Pharmakon)
- Plate readers, washers, dispensers, and liquid-handlers may be used offline to support any project that may benefit from automation
If research supported by the High-Throughput Screening Core results in publication, please acknowledge this support by including the following in your publication(s):
We thank the Alvin J. Siteman Cancer Center at Washington University School of Medicine and Barnes-Jewish Hospital in St. Louis, Mo., for the use of the High-Throughput Screening Core, which provided __________ service. The Siteman Cancer Center is supported in part by an NCI Cancer Center Support Grant #P30 CA91842.